Code:
BIOL-300
Semester:
E Semester
Course Type: Obligatory
Course website: Course Website
Course units:
2
ECTS units
3
Hours per exercise
Theory: 3
 
Instructors

Email: This email address is being protected from spambots. You need JavaScript enabled to view it.
Office phone number:  +30 2810 394355
Lab phone number:  +30 2810 394354
 
 

Description

Objectives of the course: The primary objective of this course is for students to (1) learn fundamental approaches for experimentally investigating cellular processes, (2) learn the theoretical foundations for the methods used, and (3) understand the applicability of the biochemical methods to realistic situations.

Course contents

  1. Function of cell membranes-ΑΤΡase activity --- A.Papadaki (3 hours)
  2. Measuring the water potential of plant cells --- A.Papadaki (3 hours)
  3. Molecular Genetics assays in yeast I --- M. Dramountani (3 hours)
  4. Molecular Genetics assays in yeast II --- M. Dramountani (3 hours)
  5. Isolation of lymphocytes from mouse spleen --- E. Kouimtzoglou (3 hours)
  6. Lymphoid and myeloid cell morphology --- E. Kouimtzoglou (3 hours)
  7. Immunization of experimental mice --- E. Kouimtzoglou (3 hours)
  8. Blood groups --- M. Dramountani (3 hours)
  9. Phagocytosis/lymphocyte response in mitogens --- E. Kouimtzoglou (3 hours)
  10. Flow Cytometry --- E. Kouimtzoglou (3 hours)
  11. Observing cell division with fluorescence microscopy --- E. Kouimtzoglou (3 hours)