Code: 
BIOL-251
Semester: 
E Semester
Course Type: 
Obligatory
Course units:
2
ECTS units
3
Hours per exercise
Laboratory: 3

 

Instructors



Professor: Garinis George
Email: This email address is being protected from spambots. You need JavaScript enabled to view it.
Office phone number:  +30 2810 391246
Lab phone number:  +30 2810 391072


 

Description

Objectives of the course: The primary objective of this course is for students to (1) learn fundamental approaches for experimentally investigating biological macromolecules, (2) learn the theoretical foundations for the methods used, and (3) understand the applicability of the biochemical methods to realistic situations.

Course contents

  1. DNA ligation and cloning --- D. Dokianakis (3 hours)
  2. Transformation of bacteria --- D. Dokianakis (3 hours)
  3. Lysis of bacterial cells expressing alkaline phosphatase --- D. Dokianakis (3 hours)
  4. Enzyme purification with ion-exchange chromatography --- D. Dokianakis (3 hours)
  5. Enzyme identification using polyacrylamide gel electrophoresis --- D. Dokianakis (3 hours)
  6. Enzyme action --- A.Papadaki (3 hours)
  7. DNA Exctraction from blood cells --- A.Papadaki (3 hours)
  8. Polymerase Chain Reaction --- M. Dramountani (3 hours)
  9. Genotyping --- M. Dramountani (3 hours)
  10. DNA Hybridization using Southern Ι --- A.Papadaki (3 hours)
  11. Immunological methodologies I --- E. Kouimtzoglou (3 hours)
  12. Immunological methodologies II --- E. Kouimtzoglou (3 hours)